Effects of Na /Ca exchanger downregulation on contractility and [Ca ]i transients in adult rat myocytes
نویسندگان
چکیده
GEORGE M. TADROS,2* XUE-QIAN ZHANG,1* JIANLIANG SONG,1 LOIS L. CARL,1 LAWRENCE I. ROTHBLUM,1 QIANG TIAN,1 JEREMY DUNN,3 JONATHAN LYTTON,3 AND JOSEPH Y. CHEUNG1,2 1Weis Center for Research and 2Department of Medicine, Geisinger Medical Center, Danville, Pennsylvania 17822; and 3Department of Biochemistry and Molecular Biology, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
منابع مشابه
Effects of Na(+)/Ca(2+) exchanger downregulation on contractility and [Ca(2+)](i) transients in adult rat myocytes.
Postmyocardial infarction (MI) rat myocytes demonstrated depressed Na(+)/Ca(2+) exchange (NCX1) activity, altered contractility, and intracellular Ca(2+) concentration ([Ca(2+)](i)) transients. We investigated whether NCX1 downregulation in normal myocytes resulted in contractility changes observed in MI myocytes. Myocytes infected with adenovirus expressing antisense (AS) oligonucleotides to N...
متن کاملEffects of sprint training on contractility and [Ca(2+)](i) transients in adult rat myocytes.
The effects of 6-8 wk of high-intensity sprint training (HIST) on rat myocyte contractility and intracellular Ca(2+) concentration ([Ca(2+)](i)) transients were investigated. Compared with sedentary (Sed) myocytes, HIST induced a modest (5%) but significant (P < 0.0005) increase in cell length with no changes in cell width. In addition, the percentage of myosin heavy chain alpha-isoenzyme incre...
متن کاملEffects of sprint training on contractility and [Ca ]i transients in adult rat myocytes
Zhang, Xue-Qian, Jianliang Song, Lois L. Carl, Weixing Shi, Anwer Qureshi, Qiang Tian, and Joseph Y. Cheung. Effects of sprint training on contractility and [Ca2 ]i transients in adult rat myocytes. J Appl Physiol 93: 1310–1317, 2002. First published July 5, 2002; 10.1152/ japplphysiol.01071.2001.—The effects of 6–8 wk of highintensity sprint training (HIST) on rat myocyte contractility and int...
متن کاملRegulation of cardiac myocyte contractility by phospholemman: Na+/Ca2+ exchange versus Na+ -K+ -ATPase.
Phospholemman (PLM) regulates cardiac Na(+)/Ca(2+) exchanger (NCX1) and Na(+)-K(+)-ATPase in cardiac myocytes. PLM, when phosphorylated at Ser(68), disinhibits Na(+)-K(+)-ATPase but inhibits NCX1. PLM regulates cardiac contractility by modulating Na(+)-K(+)-ATPase and/or NCX1. In this study, we first demonstrated that adult mouse cardiac myocytes cultured for 48 h had normal surface membrane ar...
متن کاملEffects of Overexpression of the Na-Ca Exchanger on [Ca]i Transients in Murine Ventricular Myocytes
We measured [Ca]i and [Na ]i in isolated transgenic (TG) mouse myocytes overexpressing the Na -Ca exchanger and in wild-type (WT) myocytes. In TG myocytes, the peak systolic level and amplitude of electrically stimulated (ES) [Ca]i transients (0.25 Hz) were not significantly different from those in WT myocytes, but the time to peak [Ca]i was significantly prolonged. The decline of ES [Ca]i tran...
متن کامل